Background:

S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acids KETAAAKFERQHMDS. S Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins.

Applications:

WB

Name of antibody:

S Tag

Immunogen:

Fusion protein of S

Full name:

S Tag

WB Predicted band size:

50 kDa

WB Positive control:

Recombinant S tag protein

WB Recommended dilution:

1000-5000