Background:

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development.

Applications:

ELISA, WB, IHC

Name of antibody:

MECP2

Immunogen:

Fusion protein of human MECP2

Full name:

methyl CpG binding protein 2

Synonyms：

RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

SwissProt:

P51608

ELISA Recommended dilution:

1000-2000

IHC positive control:

Human esophagus cancer and Human brain

IHC Recommend dilution:

100-300

WB Predicted band size:

52 kDa

WB Positive control:

Mouse lung tissue

WB Recommended dilution:

200-1000