Background:

ZsYellow1 has a true yellow emission, making it ideal for multicolor applications. Like all of Clontech's Living Colors Fluorescent Proteins, ZsYellow1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays. The gene for ZsYellow1 has been human codon-optimized to enhance its translation in mammalian cells, and adapted for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours).

Applications:

WB

Name of antibody:

ZsYellow1

Immunogen:

This antibody is raised against a recombinant protein and recognizes ZsYellow1.

WB Predicted band size:

26 kDa

WB Positive control:

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZsYellow1 ne) cDNA for 48 hrs and lysed

WB Recommended dilution:

1000-5000